Hydrogen can be used as a clean fuel in future. Photoproduction of hydrogen by anoxygenic photosynthetic bacteria is efficient and ecofriendly. A study of hydrogen production (ml/ 10 ml vessel) was carried out using anoxygenic phototrophic consortium isolated from pharmaceutical industrial with different carbon and nitrogen sources and various growth factors. The carbon source mannitol could induce a maximum of 2.5± 0.2 ml hydrogen followed by 2.0 ± 0.2 ml in benzoate, 2.0 ± 0.3 ml in acetate and 1.5 ± 0.3 in cellobiose containing medium. Ammonium chloride as nitrogen source could induced maximum of 3.0 ± 0.2 ml hydrogen and alanine induced lowest amount of hydrogen (I.0 ± 0.4 ml)by the photosynthetic consortium. Other nitrogen sources thiourea induced 2.5 ± 0.1 ml, valine 2.5 ± 0.2 ml and sodium nitrate 1.5 ± 0.2 ml of hydrogen. Of the growth factors tested, cyanocobalamine could induce highest amount of hydrogen (3.5 ± 0.1 ml) and niacin induced lowest amount (2.5 ± 0.4 ml) of hydrogen production. The growth factor riboflavin induced 3.0 ± 0.2 ml of hydrogen. Addition of growth factors showed an increase in hydrogen production by the phototrophic consortium. Highest production was seen in MAC medium at 2000lux at 1.5% concentration.