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Antimicrobial assay was done for screening purpose for all the selected gram positive and gram negative microorganisms which showed zone of inhibition against test plant extract of Aegle marmelos (Rutaceae). The two plant extracts (fruit, leaf) showed antibacterial activity against all the used bacterial strains. Maximum zone of inhibition was observed against Roultella plantikola (11 mm). Minimum zone of inhibition was observed against Pseudomonas aeruginosa by using fruit extract (7 mm). Inhibition zones of 11 mm and 9 mm were observed by using leaf and fruit extract against Roultella plantikola. The plant extract showed maximum zone of inhibition (18mm)activity against fungal strains viz.Penicillium chrysogenum and minimum (7mm)against Candida albicans. The ability of the plant extracts of Aegle marmelos to inhibit growth of bacteria and fungi is an indication of its broad spectrum antimicrobial activity which could be a potential source for development of novel antimicrobial agents.